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SARS-CoV-2 Differentiation Kit

Original SARS-CoV-2 virus or variant?

Efficient solution for screening positive samples in a single PCR run.

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SARS-CoV-2 Differentiation Kit

ViroQ® SC2 Variant 1 / ViroQ® SC2 Variant 2 Kit

Fast screening with the ViroQ® SC2 Variant Kits

Detect variants securely in just one PCR run!

Several mutations of the SARS-CoV-2 corona virus that have already been identified will have unforeseeable consequences in the pandemic. Genetic changes in the spike protein are suspected to lead to a higher risk of infection, different courses of the disease, and resistance to vaccines.

Therefore, internationally stated goals are to identify deviations from the original virus and individuals infected with the variants as quickly as possible.

To assist in this process, we have developed the ViroQ® SC2 Variant 1 and ViroQ® SC2 Variant 2 Kits. In contrast to gene-sequencing, which takes about 30 hours, the new real-time PCR assay provides results on the most important variants in only 60 minutes.

ViroQ® SC2 Variant 1

WHO Label Pango lineage
Alpha B.1.1.7
Beta B.1.351
Gamma P.1
Zeta P.2
- B.1.1.28


ViroQ® SC2 Variant 2

WHO Label Pango lineage
Alpha B.1.1.7
Delta B.1.617.2
Epsilon B.1.427 / B.1.429
Eta B.1.525
Kappa B.1.617.1
- B.1.617.3


Both Kits also included a confirmatory test for the original SARS-CoV-2 variant (Wuhan).

Thanks to screening with ViroQ® SC2 Variant 1 and ViroQ® SC2 Variant 2, laboratories can now perform gene sequencing much more effectively. Depending on the objective of the analysis, only the required samples are used. For example, if the aim is to investigate whether and how B.1.1.7 is evolving, only samples of the required variants can be sequenced.

"Why the ViroQ® SC2 Variant Kit? Unlike gene sequencing, which takes about 30 hours, ViroQ® SC2 Variant 1 and 2 detects the best-known variants in just a 60-minute PCR run. Patients infected with it can thus be identified and informed very quickly to prevent spread."

Responsibility conceived in economic terms

Whether you do your own sequencing or only test collected swabs for the virus with PCR, we all have a responsibility to use the necessary resources (hardware, software, personnel) in the best possible way to face the global pandemic. Therefore, an economically viable model is needed that is working for the laboratories as well.

Whether and to which amount the costs for sequencing and / or screening are covered depends on the respective regulations of the ministries of health of the individual countries.

  • Rapid detection of variants – sources of infection can be quickly contained
  • pre-selection of positive samples increases qualitative output in sequencing
  • higher performance – spares resource with 60 minutes PCR vs. 30 hours sequencing
  • higher efficiency – only samples with variants are sequenced
  • Safe handling, ready-to-use reagents
  • Save logistics costs – transport ViroQ® at ambient temperature

Fast One-Step Real-time PCR

Our test kits are designed for the specific detection of point mutations (SNPs) of the different mutation variants (see chart above) from throat/nose swabs. In our test kits, the RNA is converted to cDNA in a one-step PCR procedure. This cDNA is amplified in the same real-time PCR protocol.

The kit consists of the following components:

1. tube Mastermix: transcription + amplification
2. tube Reconstitution buffer for lyophilized mastermix
3. +4 tube Primer-probe mixture

Simple workflow

We have adapted our testing entirely to your needs: Simple and fast! Get results in just over 60 minutes with our ready-to-use standard reagents. The one-step PCR run (incl. reverse transcription) in the real-time cycler delivers rapid results.

More information about our rapid test:

You can find more information about COVID-19 and about our test kits in our FAQs. Here we answer questions about workflow, health measures, test kits and the evaluation procedure.

Evaluation

The four fluorescent dyes used are compatible with the common real-time cyclers – tested on QuantStudio 6 (Thermo Fischer Scientific), CFX 96TM (Bio-Rad) and Lightcycler® 480 (Roche).

The evaluation is done by the software of the hardware manufacturer.

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