Ready-to-use reagents and the real-time PCR method give you fast results. If you use our kits with FastQ® direct, you can dilute your blood sample with A. dest (1:50) and start the PCR directly. No special pre-treatment such as time-consuming heating of the sample is necessary. The PCR run takes approx. 60 minutes when using DNA and approx. 90 minutes for blood samples.
The PCR kit uses different fluorophores that are compatible with most common real-time cyclers. We have carried out tests on the following devices, among others:
- Lightcycler® 480 (Roche)
- QuantStudio 6 (Thermo Fischer Scientific)
- CFX 96TM (Bio-Rad)
The evaluation is done via the software of the hardware manufacturer.