FastQ® real-time PCR line - the fast diagnostics
Human Genetics real-time PCR typing
FastQ® real-time PCR

One product line - great variety! Our new FastQ® line offers an elegant solution for typing disease associations (ankylosing spondylitis et al., coeliac disease and haemochromatosis). The entire FastQ® line is developed for both DNA and blood samples as starting material - the choice is yours! Both variants use the advantages of real-time technology and multiplex PCR for fast and reliable typing.

  • Flexible: the liquid format is compatible with various thermal cyclers
  • Combinable: one PCR programme for all FastQ® kits, or FastQ® direct kits
  • Efficient: use the diluted blood immediately with our FastQ® direct kits
  • Safe: gel-free and without toxic residues

Reduce hands-on time to a minimum and generate clear results with our kits. We are happy to help you validate new real-time systems.

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  • FastQ® B*27
    real-time PCR kit
  • 96 tests
  • 728208
  • CE - Sign
  • Cycler Compatibility Check
    Cycler Compatibility Check
  • FastQ® B*27 direct
    Real time PCR Kit directly from blood
  • 96 tests
  • 728201
  • CE - Sign
  • Cycler Compatibility Check
    Cycler Compatibility Check
  • FastQ® CD
    Real-time PCR test
  • 48 tests
  • 728202
  • CE - Sign
  • Cycler Compatibility Check
    Cycler Compatibility Check
  • FastQ® HFE
    +++ Coming soon +++
  • 48 tests
  • 728206
  • FastQ® HFE direct
    +++ Coming soon +++
  • 48 tests
  • 728207
  • Ready-to-use reagents and the real-time PCR method give you fast results. If you use our kits with FastQ® direct, you can dilute your blood sample with A. dest (1:50) and start the PCR directly. No special pre-treatment such as time-consuming heating of the sample is necessary. The PCR run takes approx. 60 minutes when using DNA and approx. 90 minutes for blood samples.

    The PCR kit uses different fluorophores that are compatible with most common real-time cyclers. We have carried out tests on the following devices, among others:

    • Lightcycler® 480 (Roche)
    • QuantStudio 6 (Thermo Fischer Scientific)
    • CFX 96TM (Bio-Rad)

    The evaluation is done via the software of the hardware manufacturer.

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    Blood Booster for reproducible results directly from blood

    The FastQ® direct product line incorporates our new Blood Booster reagent. This ensures reliable and highly reproducible results even with difficult blood samples (e.g. high EDTA concentration) - supporting data can be found here.

    With the Blood Booster, blood samples can be diluted and pipetted directly! It saves a lot of time, work and expensive material.

    The FastQ® direct kits offer your laboratory important advantages:

    • no time-consuming pre-treatment of the blood sample
    • expensive DNA isolation is not necessary
    • reduced risk of cross-contamination
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    YOUR BENEFITS
    • the liquid format is compatible with various thermal cyclers
    • one PCR programme for all FastQ® kits, or FastQ® direct kits
    • immediate use of diluted blood with our FastQ® direct kits
    • safe handling without gels and toxic waste

    Reliably typify disease associations

    Morbus Bechterew et al

    • all common and well documented B*27 alleles covered in one PCR mix
    • discrimination of the non-associated alleles (B*27:06, B*27:09) in FastQ B*27
    • Internal amplification control included
    • genomic DNA (FastQ B*27) or whole blood / buffy coat (FastQ B*27 direct) as sample material

    Coeliac disease

    • FastQ® CD / CD direct detects the relevant DQ alleles associated with coeliac disease in only two PCR mixes using four colours
    • FastQ® CD plus / CD plus direct enables the direct detection of DQB1*03:01 and the determination of DQ2 homozygosity in only one additional mix
    • Internal amplification control included
    • genomic DNA or whole blood / buffy coat as sample material

    Hemochromatosis (HFE)

    • most common congenital disease in Northern Europe
    • multiplex real-rime PCR for hereditary hemochromatosis
    • detects the mutations H63D and C282Y for the HFE-Gen
    Amplification Disease test-kits
    Amplification from DNA M. Bechterew et al.
    Coeliac disease
    Hemochromatosis
    FastQ® B*27
    FastQ® CD
    FastQ® HFE +++coming soon +++
    Amplification from whole blood M. Bechterew et al.
    Coeliac disease
    Hemochromatosis
    FastQ® B*27 direct
    FastQ® CD direct +++coming soon+++
    FastQ® HFE direct +++coming soon+++
    Kira Kirchgessner
    Product Manager
    Blood Group Diagnostics
    +49 6404 925-318
    +49 170 9509943
    Kira Kirchgessner, Product Manager
    Beginn eines Zitats

    Thanks to our FastQ® direct kits, you can use diluted blood samples immediately. No special pre-treatment is necessary. The risk of mix-ups during DNA isolation is reduced. You also avoid environmentally harmful residues.

    Abschluss eines Zitats

    Evaluation made easy

    Fast and valid results

    Reduce hands-on time to a minimum and generate unambiguous results with our kits. We are happy to help you validate new real-time systems.

    Through optimised multiplexing and the use of Taqman® probes, we maximise the precision and safety of the FastQ® product line. For reliable results, an internal amplification control is included in every PCR set-up.

    Convenient evaluation

    Use our free PlexTyper® software to automatically analyse your real-time data. Use the worklist function via Excel to create tests for several samples. Edit and interpret your analysis easily and intuitively with the corresponding integrated tools.

    Customised hardware in a value package

    Reliable and fast typing results are provided by the combination of our FastQ® real-time PCR kits and the powerful amplification systems from Bio-Rad.

    You receive the complete test system (test kits and CFX PCR Cycler) from us, perfectly matched to each other - everything from a single source and at exciting conditions. Instruction and initial installation are also included.

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